| | Category | BI | L07 | CACT and VNTR: Is There a Linkage |
| | Abstract | The purpose of this science fair project was to see whether there is |
| | linkage between VNTR and the deletion of CACT in Intron 8 of the CD72 |
| | gene. This is relevant because the deletion of CACT can be linked to |
| | kidney cancer. |
| | |
| | My hypothesis for this project was that there is no linkage between VNTR |
| | and the deletion of CACT. |
| | |
| | To do this experiment, polymerase chain reaction (PCR) was used to |
| | amplify DNA samples at the region of the deletion. Then, restriction |
| | enzymes were used to cut samples at the CACT sequence if it exists. In |
| | this way, DNA strands with CACT were shorter. Then, gel |
| | electrophoresis was used to sort DNA strands based on strand length. In |
| | this way, one can tell whether there is a deletion of CACT or not. To see if |
| | the samples have one or two repeats of the tandem repeat, the samples |
| | were amplified using PCR. As samples with two repeats are longer, gel |
| | electrophoresis was used to sort strands based on strand length to tell the |
| | number of repeats. Twenty-two samples were used in this experiment. |
| | |
| | Statistical tests were used to analyze the data. a t-value of .,711 was |
| | derived from a correlation coefficient. Howevever, the critical value for a |
| | 95% confidence level is 2.2325. It was conclueded that there was no |
| | statistically significant linkage. |
| | |
| | In the future,one could do this project with more samples. One might |
| | compare the probability to have a deletion of CACT in this intron between |
| | different groups of people for a future project. |
| | |
| | Bibliography | "DNA Fingerprinting, Genetics and Crime: DNA Testing and the Courtroom." |
| | Fathom :: The Source for Online Learning. N.p., n.d. Web. 30 Nov. 2009. |
| | <http://www.fathom.com/course/21701758/session1.html>. |
| | |
| | Devore, Jay L.. Probability and Statistics for Engineering and the |
| | Sciences/Book and Disk. 4th/Bk&Dsk ed. New York: Brooks/Cole Pub Co, |
| | 1995. Print. |
| | "Electrophoresis/Agarose Gel Electrophoresis Protocols." Protocol |
| | Online - Your lab's reference book . N.p., n.d. Web. 30 Nov. 2009. |
| | <http://www.protocol- |
| | online.org/prot/Molecular_Biology/Electrophoresis/Agarose_Gel_Electropho |
| | resis/index.html>. |
| | |
| | "Generation & Maintenance of Linkage Disequilibrium." Guide to Genetic |
| | Analysis. N.p., n.d. Web. 5 Feb. 2010. |
| | <http://slack.ser.man.ac.uk/theory/ld_gen.html>. |
| | |
| | Hitomi, Yuki. " CD72 polymorphisms associated with alternative |
| | splicing modify susceptibility to human systemic lupus erythematosus |
| | through epistatic interaction with FCGR2B -- Hitomi et al. 13 (23): 2907 -- |
| | Human Molecular Genetics." Oxford Journals | Life Sciences & Medicine | |